LOCUS       40924_485        7822 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Integrating vector CIpNAT plasmid CIpNat, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Shahana S, Childers DS, Ballou ER, Bohovych I, Odds FC, Gow NA, 
            Brown AJ.
  TITLE     New Clox Systems for Rapid and Efficient Gene Disruption in Candida 
            albicans
  JOURNAL   PLoS ONE 9 (6), E100390 (2014)
  PUBMED    24940603
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Shahana S, Childers DS, Ballou ER, Bohovych I, Odds FC, Gow NA, 
            Brown AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2014) School of Medical Sciences, University of 
            Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25
            2ZD, UK
REFERENCE   3  (bases 1 to 7822)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7822)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2014"; volume: "9"; issue: "6"; pages: "E100390"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2014) School of Medical Sciences, University of Aberdeen, 
            Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_recomb     1040..1513
                     /label=RPS1 targeting sequence
                     /note="RPS1 targeting sequence"
     primer_bind     complement(1511..1527)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1544..1560
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1562..1595
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     promoter        1636..1979
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1999..2562
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     terminator      2580..2777
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     regulatory      complement(2810..3206)
                     /note="Candida albicans ACT1 terminator (Staib et al.,
                     1999)"
                     /regulatory_class="terminator"
     CDS             complement(3207..4475)
                     /label=FLP
                     /note="site-specific recombinase"
     regulatory      complement(4500..5542)
                     /gene="SAP2"
                     /note="Candida albicans SAP2 promoter (Staib et al., 1999)"
                     /regulatory_class="promoter"
     gene            complement(4500..5542)
                     /gene="SAP2"
                     /label=SAP2
     primer_bind     5554..5570
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(5572..5605)
                     /note="FLP recombination target sequence (FRT) (Staib et
                     al., 1999)"
     protein_bind    5572..5605
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     promoter        complement(5638..5656)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5677..5693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5701..5717)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5724..5754)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5769..5790)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6078..6666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6840..7697)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7698..7802)
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct
      661 ctcgcgtaga tccaactcaa gtacaacatg gctgtcggta aaaacaagag attgtccaaa
      721 ggaaagaaag gattaaaaaa gaaggtcgtt gacccattca ccagaaaaga ttggtttgac
      781 atcaaagctc caaccacttt tgaaaacaga aatgttggta aaactttgat caacagatct
      841 actggtttaa agaatgccgc tgatggcttg aaaggtagag ttttcgaagt ttgtttggcc
      901 gacttacaag gttccgaaga ccactcttac agaaaaatca aattgagagt tgatgaagtc
      961 caaggtaaaa acttgttgac caacttccat gaggcctcca tgcaggcctc atggaggcct
     1021 catggaggcc tcatggaggc cccgggaaat taagatcatt ggtcagaaaa tggcaatcat
     1081 tagttgaagc taatgtcact gttaaaactt ccgacgatta cgttttgaga gtttttgcca
     1141 ttgctttcac caaaagacaa ccaaaccaaa tcaagaaaac tacttacgct caatcctcta
     1201 aattgagaga agtcagaaag aagatgattg aaattatgca aagagaagtt tccaactgta
     1261 ctttagctca attaacttcc aaattgattc cagaagtcat tggccgtgaa attgaaaaat
     1321 ccacccaaac cattttccca ttacaaaatg tccacatcag aaaagtcaaa ttgttgaaac
     1381 aaccaaaatt cgacttgggt tcattattgg ctttgcacgg tgaaggttca accgaagaaa
     1441 aaggtaagaa agtttcttct ggtttcaaag atgttgtttt agaatctgtt taaggataag
     1501 gatctggggg gatccactag ttctagaccg cggtggcggc cgctctagaa ctagtggatc
     1561 tgaagttcct attctctaga aagtatagga acttccatca agcttgcctc gtccccgccg
     1621 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
     1681 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa
     1741 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg
     1801 ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc
     1861 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata
     1921 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaaccg
     1981 gatcctacaa ttatgtctac tactttggat gatactgctt atagatacag aacttctgtt
     2041 ccaggtgatg ctgaagctat tgaagctttg gatggttctt tcactaccga tactgttttt
     2101 agagttactg ctactggtga tggtttcact ttgagagaag ttccagttga tccaccattg
     2161 actaaggttt tcccagatga tgaatccgat gatgaatccg atgctggtga agatggtgat
     2221 ccagattcta gaactttcgt tgcttatggt gatgatggtg atttggctgg tttcgttgtt
     2281 gtttcttatt ctggttggaa cagaagattg actgttgaag atattgaagt tgctccagaa
     2341 catagaggtc atggtgttgg tagagctttg atgggtttgg ctactgaatt cgccagagaa
     2401 agaggtgctg gtcatttgtg gttggaagtt accaatgtta atgctccagc tattcatgct
     2461 tatagaagaa tgggtttcac tttgtgtggt ttggatactg ctttatacga tggtactgct
     2521 tccgatggtg aacaagcttt gtatatgtct atgccatgtc cataataact cgagtcagta
     2581 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
     2641 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     2701 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     2761 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg tcgacatttt
     2821 atgatggaat gaatgggatg aatcatcaaa caagagaaaa tacccgtgac gaaaataata
     2881 aaataagttc ctctgataca gaagatgaaa acaacaacaa caagatatag aaatgccttg
     2941 ggtggctatt ttatagtctt aactttttaa tgtatatttg ttttgttttt ttacataata
     3001 atactttata aaagctaagc taaattcaag taaaatatca atctctcaaa taaaacattt
     3061 ttctcttttt cttaaattta gttttatata tttataaaat aaacaaagat ttttttaaaa
     3121 aagtaacaag ttatatatgt aataacaaaa agaagaataa caagaataca aaaccagatt
     3181 tccagatttc cagaatttca ctcttatatg cgtctattta tgtaggatga aaggtagtct
     3241 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
     3301 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
     3361 atcatttcct ttgatattgg atcatatgca tagtaccgag aaactagtgc gaagtagtga
     3421 tcaggtattg ctgttatctg atgagtatac gttgtcctgg ccacggcaga agcacgctta
     3481 tcgctccaat ttcccacaac attagtcaac tccgttaggc ccttcattga aagaaatgag
     3541 gtcatcaaat gtcttccaat gtgagatttt gggccatttt ttatagcaaa gattgaataa
     3601 ggcgcatttt tcttcaaagc tttattgtac gatctgacta agttatcttt taataattgg
     3661 tattcctgtt tattgcttga agaattgccg gtcctattta ctcgttttag gactggttca
     3721 gaattcctca aaaattcatc caaatataca agtggatcga tcctacccct tgcgctaaag
     3781 aagtatatgt gcctactaac gcttgtcttt gtctctgtca ctaaacactg gattattact
     3841 cccaaatact tattttggac taatttaaat gatttcggat caacgttctt aatatcgctg
     3901 aatcttccac aattgatgaa agtagctagg aagaggaatt ggtataaagt ttttgttttt
     3961 gtaaatctcg aagtatactc aaacgaattt agtattttct cagtgatctc ccagatgctt
     4021 tcaccctcac ttagaagtgc tttaagcatt tttttactgt ggctatttcc cttatctgct
     4081 tcttccgatg attcgaactg taattgcaaa ctacttacaa tatcagtgat atcagattga
     4141 tgtttttgtc catagtaagg aataattgta aattcccaag caggaatcaa tttctttaat
     4201 gaggcttcca aaattgttgc tttttgcgtc ttgtatttaa actggagtga tttattgaca
     4261 atatcgaaac tcaacgaatt gcttatgata gtattatagc tcatgaatgt ggctctcttg
     4321 attgctgttc cgttatgtgt aatcatccaa cataaatagg ttagttcagc agcacataat
     4381 gctattttct cacctgaagg tctttcaaac ctttccacaa actgacgaac aagcacctta
     4441 ggtggtgttt tacataatat atcaaattgt ggcatgtcga caatggtgat gtctagtggg
     4501 ttgttgttat ttgattgatt aattgattga tattattcaa attttcaaca accaagagga
     4561 atcaactatc cttttatact attctttttt ttgaaattag atatctgaac agattaagaa
     4621 ctagcttatt gttttttttc attctccttc tcgtaccctt ttctttttag atgtttatta
     4681 tcattgctat aaattgtggt gttctttgtt atcgttttac tatgaatgtg atctatattt
     4741 ttttagtttg atgccgttta ttcaaatgca tttcattcaa ttcaaatcaa ttcaaatcaa
     4801 tttaaattta actttatcaa ttatagatta aatttatcaa tattacccgt ttcaccttct
     4861 gcccccctcc cccggtttcc ccgatttcca ggttttgttt actaaaattt aaaaaataat
     4921 ttagttcttt tttgatgaaa gaaaaaggga aataacttct tcatttcatg attgggatta
     4981 aaacgcacgc gtgaattatt ggtactagaa agattaaaag tttagtctaa tgtattattt
     5041 tctgttttag ttgatgcatt aaacaccttg aacttttctc cattattatt ttcgaaaaat
     5101 tagagcaagt caaaaagcaa agcaaagcaa agcaaagcta gcttattccg ataatcatta
     5161 tttctatcgg aaatctattg ttgttgtcac cgtattggtt gaaatcaatt ttgtgttttg
     5221 tggtgccgtg caagtttcta tctcctcctc ctcctattcc ttctccttat ggtgtgttgt
     5281 tgacaggcaa caggagaggt tatgatttga aatttgtgtt tgttgacgtt tcaagtcaaa
     5341 ctaaaactac ttttttggat tgaaattata attgaatgat gcttagaatg attctggata
     5401 ccaagaaatt gtaaaaaagt gtttttttgt tttaaggggt aacacatttg gtgtgtgtgt
     5461 gtcaaaataa tctgtcaaaa aaataaagtc aaagttccaa ggggaaagaa aatttgaagt
     5521 ttttacttgg caatatttaa atgcttgcaa aaatctagaa ctagtggatc cgaagttcct
     5581 attctctaga aagtatagga acttcctcga ggggggcccg gtacccagct tttgttccct
     5641 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5701 ttgttatccg ctcacaatcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5761 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5821 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5881 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5941 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6001 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6061 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6121 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6181 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6241 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6301 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6361 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6421 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6481 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     6541 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6601 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6661 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6721 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6781 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6841 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6901 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6961 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7021 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7081 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7141 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7201 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7261 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7321 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7381 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7441 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7501 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7561 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7621 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7681 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7741 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7801 cgcacatttc cccgaaaagt gc
//
